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SUNDARAM RAMAKRISHNAN
Address
3267 Lanier Place
Thousand Oaks
CA, 91360
Phone    : (805) 492 – 6535 (Home)
Fax        : (805) 492 – 0908
E – mail: ram@cimm.net
Education
Ph. D. 1991(Biotechnology) Imperial College (University of London), London, UK.“Construction and properties of lactose fermenting brewers’ and bakers’ yeast”Supervisor: Prof. B.S. Hartley, FRS
M. Phil. 1982(Biology)  Madurai Kamaraj University, Madurai, India.“DNA-binding proteins of chick embryonic brain”
M. Sc. 1981(Microbiology) Madurai Kamaraj University, Madurai, India.“Influence of Azotobacter chroococcum on growth and yield of paddy”
B. Sc. 1979  Madras University, Madras, India.Zoology, chemistry and Entomology
Summer 1993
Summer 1993 Spetsai Summer School on “Protein Structure, Function and Design” (NATO).
Summer 1987  Engineering Principles for Biologists, Indian Institute of Technology, New Delhi, India.
Awards: American Heart Association Post-Doctoral Fellowship, 1993 -1995
British Commonwealth Fellowship, 1987 – 1991
NATO grant to participate Spetsai Summer School, 1993
Experience:
July 99 toPresent Senior Scientist, California Institute of Molecular Medicine
June 95 to Sept. 97 Staff Research Associate, Protein Expression Laboratory, Department of Structural Biology and Molecular Medicine, Molecular Biology Institute, University of California, Los Angeles, CA 90023.“Expression, Purification and characterization of Recombinant Proteins expressed in E.coli and yeast:
June 91 to Sept. 91 Academic Visitor, Center for Biotechnology, Imperial College, London. Collaborated with Whitbread Breweries, UK, in transferring my Ph.D work.
Sept. 83 to Sept. 89 Assistant Professor, Department of Microbiology, PSG College of Arts and Science, Coimbatore, India.
May 82 to Sept. 83 Junior Superintendent, Central Warehousing Corporation of India.
    Technical Expertise:

    Protein chemistry and Molecular Biology
     

  • Construction and Screening of cDNA libraries, Cloning, Sequencing, Northern, Southern and Western blots and PCR (cloning, mutagenesis etc.)
  • Expression, optimization and purification of recombinant proteins in E. coli using pET, pTricHis, pMal(maltose binding protein) and GST fusion systems
  • Novel expression systems to obtain soluble protein, chaperonin mediated (GroEL, GroES) in vivo expressions and thioredoxin co-expression
  • Optimization protease cleavage of fusion proteins and purification
  •  Purification of proteins by column chromatography (ion-exchange, affinity and hydrophobic), HPLC (reverse phase, gel filtration), BioRad Economy and Biologic system (FPLC)
  •  Designing and construction of novel proteins by site-directed mutagenesis.
  •  Protein expression using Pichia pastoris and Saccharomyces cerevisea
  •  Physical analysis of proteins (Circular Dichroism, Analytical Gel Filtration, Fluorescence Spectroscopy), Enzyme Kinetics
     
    Microbiology and Genetics
     
  •  Physiological studies of recombinant microorganisms using bench-top bioreactors (aerobic and anaerobic conditions) 
  • High density microbial fermentation for recombinant protein production (Fed-batch)
  •  Chromatographic quantitation of sugars and alcohol
  • Prokaryotic (E.coli) and eukaryotic (yeast) genetical techniques
    Research Publications:

    Ramakrishnan, S., and Hitchcock-DeGregori, S. E. (1996).  Structural and Functional Significance of Asp 89 of Troponin C central helix. Biochemistry 35: 15515-15521

Ramakrishnan, S., Hong, J., Kaufman, D, Middleton, B., Radhakrishman, S., and Perry, L. J. (1996). Expression of human glutamic acid decarborxylase in Pichia pastoris, Current topics in gene expression systems, page 57 (abs)

Perry, L. J., Han, A., Ramakrishnan, S., Heckmann, K., and Weiss, M. S. (1996). E.coli expression, purification and crystallization studies of Euplotes octocarinatus pheromones 2 and 3, Association of Biomolecular Resource Facilities: Biomolecular Techniques (abs)

Ramakrishnan, S., and Hitchcock-DeGregori, S. E. (1995). Investigation of the structural requirements of the troponin C central helix for function, Biochemistry, 34: 16789-96

Ramakrishnan, S., and Hitchcock-DeGregori, S. E. (1994). Significance of the length of the troponin C central helix. Biophys. J. 66: 309A

Ramakrishnan, S., and Harley, B.S. (1993). Fermentation of lactose by yeast cells secreting recombinant fungal b-galactosidase. Applied and Environmental Microbiology 59: 4230-35

Ramakrishnan, S., and Hitchcock-DeGregori, S.E. (1993). Consequence of insertion in and deletion of the central helix region of troponin C. Biophys. J. 64: 134A

Kumar, B., Ramakrishnan, S., Teri, T. T., J.K. C., and Hartley, B. X. (1992). Saccharomyces cerevisiae cells secreting an Aspergillus niger b-galactosidase grow on whey permeate. Biotechnology 10: 82-85

Hartley, B. S., Kumar, V., and Ramakrishnan, S. (1990). DNA construct and Modified Yeast UK Patent PCT/GB/90/00373

Referees:

Available upon request

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